Thermo scientific revertaid first strand cdna synthesis kit, 100 reactions, price. The kit includes revertaid h minus reverse transcriptase, which. Nzy firststrand cdna synthesis kit is a system that includes all the necessary components to synthesize first strand cdna, except the template rna. Random hexamer primers do not require the presence of the polya tail, therefore, they can be used for transcription of the 5end regions of mrna or cdna. Oligodt 15 primer vial 5 for standard cdna synthesis. Researchers can choose from among a wide variety of commercial cdna synthesis kits. Download a pdf containing pricing for our full product list. Reverse transcriptases rts use an rna template and a primer complementary to the rna to direct the synthesis of the first strand cdna. Thermo scientific reverse transcription thermo fisher. The revertaid premium doublestranded cdna synthesis kit is a complete system for efficient synthesis of doublestranded cdna from total rna or mrna. Some are designed strictly for first strand cdna synthesis. This technique can be used to clone members of a multigene family, homologous genes from different species, and genes previously identified. For your convenience, reagents are available as kits or standalone products, depending on your needs. The kit uses mmulv vity compared to amw reverse transcriptase.
First strand cdna synthesis procedure the first strand cdna reaction can be performed as an individual reaction or as a series of parallel reactions with different rna templates. Primers are not included in the preassembled first strand. The revertaid first strand cdna synthesis kit is supplied with both oligodt 18 and random hexamer primers. The thermo scientific revertaid first strand cdna synthesis kit is a complete system for efficient synthesis of first strand cdna from mrna or total rna templates. The oligodt 18 primer anneals selectively to the polya tail of mrna.
The transcriptor first strand cdna synthesis kit is designed to reverse transcribe rna mrna, total rna, viral rna, and in vitrotranscribed rna from a variety of sources for the following applications. The cdna synthesis revertaid h minus first strand cdna synthesis kit. Nzy firststrand cdna synthesis kit cdna kits nzytech. Revertaid 42 c separate components 20 reactions 93. The thermo scientific revertaid rt kit is a complete system for efficient synthesis of first strand cdna from mrna or total rna templates. Revertaid first strand cdna synthesis kits fermentas.
Thermo scientific revertaid first strand cdna synthesis kit. Firststrand cdna synthesis kit ge healthcare, 27926101. Thermo scientific revertaid rt reverse transcription kit. Immunomodulation of surface biofunctionalized 3d printed. The monsterscript 1st strand cdna synthesis kit has been discontinued. Nzy firststrand cdna synthesis kit is formulated to provide high yields of fulllength cdna. The kit is suitable for synthesis of cdna up to kb. Thermo scientific first strand cdna synthesis kit is a complete system for efficient synthesis of first strand cdna from mrna or total rna templates.
Ribolock rnase inhibitor, supplied with the kit, effectively protects rna templates from degradation. Reverse transcription rt is a widely used application in molecular biology research for rna and expression analysis. Avian myeloblastosis viral amv reverse transcriptase rt was the first enzyme used to synthesize cdna. Monsterscript reverse transcriptase is a thermostable reverse transcriptase that completely lacks rnase h activity. The first strand of cdna can be directly used as a template in pcr see rtpcr protocol or in second strand cdna synthesis see protocol. First strand cdna synthesis kit direct pcr reagents and.
It uses cloned moloney murine leukemia virus mmlv reverse transcriptase to synthesize first strand cdna. The kit includes revertaid h minus reverse transcriptase, which has a point mutation that completely eliminates rnase h activity. Amplification of cdna by pcr is an essential tool for todays molecular biologist. The enzymes lack of rnase h activity contributes to its ability to make longer cdnas and more complete fulllength libraries of first strand cdna.
These reagents should remain stable for at least 1 year in their unopened containers. Substrate mechanics dictate cellcell communication by gap. Genespecific primers may also be used with the kits. The enzyme maintains activity at 4250c and is suitable for synthesis of cdna up to kb. Reverse transcriptase, which has lower rnase h acti first. Liscript first strand cdna synthesis kit contains all the components necessary for the 1st strand cdna synthesis. Discriminating between the mrna and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. Thermo scientific revertaid first strand cdna synthesis. Neb offers several reagents for cdna synthesis for use in applications including qpcr and qrtpcr.
Protoscript first strand cdna synthesis kit features two optimized mixes. Truscript first strand cdna synthesis kit for mrna product. First strand cdna synthesis kit is designed to generate fulllength first strand cdna from mrna templates. The kit uses revertaid reverse transcriptase rt, which has lower rnase h activity compared to amv reverse transcriptase. Revertaid h minus first strand cdna synthesis kit is a complete system for efficient synthesis of first strand cdna from rna templates. Revertaid first strand cdna synthesis kit fermentas and the last, said to be the best but not explained to me why it is so smarter race cdna amplification kit clontech. The specific gene reagents included the mrna extract kit the rneasy plus mini kit, no. Thermo scientific revertaid rt kit is a complete system for efficient synthesis of first strand cdna from mrna or total rna templates. First strand cdna synthesis was carried out with 1x mmulv.
First strand cdna synthesis kit for rtpcr amv protocol. Revertaid 42 c separate components 100 reactions 379. The resulting singlestranded cdna is suitable for use in realtime quantitative reverse transcription pcr rtqpcr. Amv reverse transcritase is used for first strand cdna synthesis. Both kits are suitable for synthesis of cdna as long as kb. It can be used with either sequencespecific primers, polydt 15 primers, or random primers.
Add the following components in the indicated order. Thermo scientific revertaid h minus first strand cdna. The transcriptor high fidelity cdna synthesis kit is designed to reverse transcribe rna with an increased fidelity cat. Nebnext ultra ii rna first strand synthesis module neb.
The thermo scientific reverse transcription portfolio offers robust and reliable. The first strand cdna product generated is up to 10 kb. The nebnext ultra ii rna first strand synthesis module has been optimized to convert a broad range of input amounts of rna into cdna. First strand cdna synthesis kit for rtpcr amv sigma. Thermo scientific revertaid premium first strand cdna. Thermo scientific maxima h minus doublestranded cdna. Thermo scientific revertaid first strand cdna synthesis kit is a complete system for efficient synthesis of first strand cdna from rna templates. Norgens truscript first strand cdna synthesis kit for mrna components should be stored at 20oc.